Length 2 1/2 – 3 3/4 in. (6.5-9.5 cm). [Note: This species is distinguished from the Southern Two-lined Salamander through genetics and geographic location.] These are common brookside inhabitants found in all but the northwestern quarter of our state. They prefer small rocky woodland streams as well as springs and seeps, where they spend the day hiding beneath flat rocks and logs. Like the Northern Dusky Salamander, with which they often associate, two-lined salamanders are very abundant. They are easy to find but extremely difficult to catch and hold. As its name implies, the Two-lined Salamander has two dark lines, one on either side of its bright yellow or golden brown back. The Northern Two-lined Salamander (E. bislineata) is typically found north of Interstate 70, while the Southern Two-lined Salamander (E. cirrigera) occupies the southern portion of the state.
Text courtesy of the Ohio Division of Wildlife: https://wildlife.ohiodnr.gov/portals/wildlife/pdfs/publications/id%20guides/pub348.pdf